Lepstospira interrogans shotgun phage display identified LigB as a heparin-binding protein
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منابع مشابه
Novel Applications of Shotgun Phage Display
Rosander, A. 2004. Novel applications of shotgun phage display. Doctor’s dissertation. ISSN 1401-6249, ISBN 91-576-6461-7 In a shotgun phage display library, theoretically, the entire proteome of a bacterium is represented. Phages displaying specific polypeptides can be isolated by affinity selection, while the corresponding gene remains physically linked to the gene product. The overall object...
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Random peptide libraries are displayed on filamentous bacteriophage as fusions to either the minor coat protein, pIII, or the major coat protein, pVIII. We have devised a means of isolating the peptide displayed on a phage clone by transferring it to the N-terminus of the maltose-binding protein (MBP) of Escherichia coli encoded by malE. Transfer of a peptide sequence to monomeric MBP eliminate...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 2012
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2012.09.137